Primary and Stem Cells - by Uma Lakshmipathy & Bhaskar Thyagarajan Hardcover

About the Book

"This book describes basic cell engineering methods, emphasizing stem cell applications, and use of the genetically modified stem cells in cell therapy and drug discovery. Together, the chapters introduce and offer insights on new techniques for engineering of stem cells and the delivery of transgenes into stem cells via various viral and non-viral systems. The book offers a guide to the types of manipulations currently available to create genetically engineered stem cells that suit any investigator's purpose, whether it's basic science investigation, creation of disease models and screens, or cells for therapeutic applications"--Provided by publisher.

Book Synopsis

This book describes basic cell engineering methods, emphasizing stem cell applications, and use of the genetically modified stem cells in cell therapy and drug discovery. Together, the chapters introduce and offer insights on new techniques for engineering of stem cells and the delivery of transgenes into stem cells via various viral and non-viral systems. The book offers a guide to the types of manipulations currently available to create genetically engineered stem cells that suit any investigator's purpose, whether it's basic science investigation, creation of disease models and screens, or cells for therapeutic applications.

From the Back Cover

The all-important resource that lays the groundwork for researchers to genetically modify primary and stem cells for use in downstream applications

Replacing damaged or lost organs by producing "spare parts" is a concept that lies at the core of the varied biotechnological practices generally referred to as tissue engineering. An important component in this regeneration process includes the use of stem cells, which harbor the ability to mature into other cell types and hold the potential to act as a repair system for the body. Primary and Stem Cells describes basic cell engineering methods, emphasizing stem cell applications, and the use of genetically modified stem cells in cell therapy and drug discovery. Discussion includes new techniques for the engineering of stem cells and the delivery of transgenes into stem cells via various viral and non-viral systems, along with:

• Transfection techniques and methods for cell line engineering

• Information that assists researchers and drug developers in deciding how to generate their required drug or gene therapists in developing genetically modified cells

• An overview of applications in areas like disease models and drug discovery

• Issues that address the interests of companies involved in stem cell tools and reagents

Primary and Stem Cells serves as an essential guide to the types of manipulations currently available to create genetically engineered stem cells that suit any investigator's purpose --basic science investigation, creation of disease models and screens, or cells for therapeutic applications--and acts as the launching pad for pioneering further development in a field emerging with exciting possibilities.

Review Quotes

"This is an excellent resource on the new techniques for the engineering of stem cells and the delivery of transgenes into stem cells via various viral and nonviral systems. It provides comprehensive information on the concepts of cell engineering principles and their application in primary and stem cells. Researchers, established stem cell scientists, and graduate students interested in this field will find this book very handy." (Doody's, 17 August 2012)

About the Author

UMA LAKSHMIPATHY is a principal investigator at Life Technologies. She has a PhD in life sciences, with academic and industry experience in molecular biology and stem cells. Dr. Lakshmipathy holds four patents and has authored more than forty publications.

BHASKAR THYAGARAJAN is a program manager at Life Technologies. He has a PhD in pharmacology, with expertise in the areas of molecular biology, DNA recombination, gene and cell therapy, and protein purification. He holds one patent and has authored more than twenty publications.